GeneTex
United States (US)

Nectin 3 antibody [103-A1]

Cat No. GTX16913

Host Rat
Clonality Monoclonal
Clone Name 103-A1
Isotype IgG2a
Application IHC-Fr, FACS, IP, IA
Reactivity Mouse
APPLICATION

Application Note

For FACS: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For IA: Use at an assay dependent dilution. Is unsuitable for WB. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline containing 0.02% sodium azide and 0.1% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400.

Purification

Protein G affinity purified
Provided as a 0.2 um purified antibody solution

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cell Membrane

Background

Nectin (originally isolated as poliovirus related-protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin/PRR-family, also described as the alpha herpes virus receptor family consists of four members, nectin-1, -2, -3 and -4. All the members of the nectin family have two or three slice variants. For Nectin 3; Nectin 3 alpha, 3 beta and 3 gamma isoforms exist. Both nectin 2 and nectin 3 are ubiquitously expressed whereas nectin 1 is abundantly expressed in brain. Nectin 2 or 3 is expressed in cells where cadherin are mainly expressed in testis and placental tissues. Nectin 3/PRR3 interacts in vivo with both long and short isoforms of afadin.

Research Area

Package List Price ($)
$ 319