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NeuN antibody

Anti-NeuN antibody used in Western Blot (WB). GTX133127
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX133127
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

Cat No. GTX133127

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NeuN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886836

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding protein, fox-1 homolog (C. elegans) 3 , Fox-3 , Hrnbp3 , NeuN , Neuna60

Cellular Localization

Nucleus , Cytoplasm

Background

RNA-binding protein that regulates alternative splicing events.

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in Western Blot (WB). GTX133127

GTX133127 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody (GTX133127) diluted at 1:1000.

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX133127

GTX133127 IHC-P Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

GTX133127 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX133127

GTX133127 IHC-Fr Image

NeuN antibody detects NeuN protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: NeuN protein stained by NeuN antibody (GTX133127) diluted at 1:250.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169