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NeuN antibody

Anti-NeuN antibody used in Western Blot (WB). GTX16208
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208
Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

Cat No. GTX16208

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Horse
Package
100 μl ($339)

APPLICATION

Application Note

WB:1:5,000-1:10,000. IF/IHC 1:500-1:1,000.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Whole serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment corresponding to Human NeuN aa 1-100 (N terminal)

Purification

Unpurified
Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in Western Blot (WB). GTX16208

GTX16208 WB Image

Western blot of rat brain homogenates stained with GTX16208. Antibody binds closely spaced 48 and 46kDa bands.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

GTX16208 IHC-Fr Image

Paraformaldehyde fixed frozen section of adult rat cortex stained with GTX16208 in red and counterstained for DNA in blue. Note that GTX16208 stains neuronal nuclei and distal perikarya. These antibodies do not bind to the nuclei of perikarya of non-neuronal cells, so that they can be used to identify neurons.

Anti-NeuN antibody used in IHC (Frozen sections) (IHC-Fr). GTX16208

GTX16208 IHC-Fr Image

Paraformaldehyde fixed frozen section of adult rat brain stem stained with NeuN antibody in red and counterstained for MAP2 in green. DNA is shown in blue. Note that NeuN antibody stains neuronal nuclei and distal perikarya and that the MAP2 antibody stains the dendrites extending from these cells. These antibodies do not bind to the nuclei of perikarya of any non-neuronal cells, so that they can be used to identify and quantify neurons.

REFERENCE

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REVIEW

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