Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Neuroligin 1 antibody [GT63] (GTX641572) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.