Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat heart. Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(GTX105711) antibody at 1:200 dilution.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.