Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody (GTX113510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term (GTX113510) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NuMA antibody [N1], N-term detects NuMA protein at nucleus in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
NuMA antibody [N1], N-term (GTX113510) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA(GTX113510) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA (GTX113510) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NuMA antibody [N1], N-term detects NuMA protein at nucleus in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
NuMA antibody [N1], N-term (GTX113510) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min