APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:5000 |
1:10-1:100 |
Assay dependent |
Assay dependent |
Note :
ICC/IF
Suggest to fix cells with 4% paraformaldehyde and permeabilized with 0.25% Triton-X 100.
|
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
Tissue culture supernatant
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Nuclear matrix extract from MDCK cells.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C23 , NCL , nucleolin , Nucleolin
Cellular Localization
Nucleus,Nucleolus,Cytoplasm
Background
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX14966 WB Image
WB analysis of HeLa nuclear extract using GTX14966 Nucleolin antibody [4i51]. Dilution : 1:5000
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REFERENCE
There are currently no references for Nucleolin antibody [4i51] (GTX14966). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Nucleolin antibody [4i51] (GTX14966). Be the first to share your experience with this product.