ELISA: Use at an assay dependent dilution. ICC: Use at a dilution of 1/10 - 1/100. Shows positive immunostaining for nucleolin in HeLa cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X 100IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/5000. detects nucleolin in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti nucleolin (1:5000 dilution). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 85 kDa. Optimal dilutions/concentrations should be determined by the end user.
HeLa nuclear extract/ HeLa cells
Recognizes nucleolin, Mr 100kDa and an additional protein, Mr 95kDa.
Culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Nuclear matrix extract from Human MDCK cells.
Tissue culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nuclear, located mainly in dense fibrillar regions of the nucleolus.
Nucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein (this domain is responsible for the ablility of nucleolin to modulate chromatin condensation), a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin also interacts directly with DNA topoisomerase I. It is located mainly in dense fibrillar regions of the nucleolus. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. It interacts with APTX and contains 4 RNA recognition motif (RRM) domains.
GTX14966 WB Image
Western Blot analysis using Nucleolin [4i51] antibody (GTX14966) on a Hela nuclear extract