Nucleophosmin antibody

Name: Nucleophosmin antibody
Brand: GeneTex
SKU: GTX112654
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Nucleophosmin antibody used in IHC (Paraffin sections) (IHC-P). GTX112654

Anti-Nucleophosmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112654

Anti-Nucleophosmin antibody used in Western Blot (WB). GTX112654

Cat. No. GTX112654

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Nucleophosmin products

View product citations for antibody GTX112654 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Nucleophosmin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleophosmin 1 , B23 , NPM

Cellular Localization

Nucleus , nucleolus

Background

NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]

Database

Gene ID: 4869 NPM1
UniProt: P06748 NPM1

Research Area

DATA IMAGES

GTX112654 IHC-P Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nucleophosmin antibody (GTX112654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112654 ICC/IF Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleophosmin protein stained by Nucleophosmin antibody (GTX112654) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX112654 WB Image

Nucleophosmin antibody detects Nucleophosmin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nucleophosmin antibody (GTX112654) diluted at a dilution of 1:1000.

GTX112654 IHC-P Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Nucleophosmin antibody (GTX112654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.

Application : WB

Reactivity : Human

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