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Nucleophosmin antibody

Anti-Nucleophosmin antibody used in IHC (Paraffin sections) (IHC-P). GTX112654
Anti-Nucleophosmin antibody used in Western Blot (WB). GTX112654
Anti-Nucleophosmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112654
Anti-Nucleophosmin antibody used in IHC (Paraffin sections) (IHC-P). GTX112654

Cat No. GTX112654

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Nucleophosmin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nucleophosmin 1 , B23 , NPM

Cellular Localization

Nucleus , nucleolus

Background

NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Nucleophosmin antibody used in IHC (Paraffin sections) (IHC-P). GTX112654

GTX112654 IHC-P Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Nucleophosmin antibody (GTX112654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nucleophosmin antibody used in Western Blot (WB). GTX112654

GTX112654 WB Image

Nucleophosmin antibody detects Nucleophosmin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nucleophosmin antibody (GTX112654) diluted at a dilution of 1:1000.

Anti-Nucleophosmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112654

GTX112654 ICC/IF Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleophosmin protein stained by Nucleophosmin antibody (GTX112654) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Nucleophosmin antibody used in IHC (Paraffin sections) (IHC-P). GTX112654

GTX112654 IHC-P Image

Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nucleophosmin antibody (GTX112654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169