Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with OAS3 antibody (GTX118059) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using OAS3(GTX118059) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min