Wild-type (WT) and OCT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody (GTX105202) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

OCT1 antibody detects OCT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OCT1 protein stained by OCT1 antibody (GTX105202) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using OCT1(GTX105202) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 μg of OCT1 antibody (GTX105202).
Western blot analysis was performed using OCT1 antibody (GTX105202).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody (GTX105202) diluted at 1:1000.

OCT1 antibody immunoprecipitates OCT1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 μg of OCT1 antibody (GTX105202) 7.5% SDS-PAGE The immunoprecipitated OCT1 protein was detected by OCT1 antibody (GTX105202) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

OCT1 antibody detects OCT1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
OCT1 antibody (GTX105202) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min