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OPG antibody

Overexpression
Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX127948
Anti-OPG antibody used in Western Blot (WB). GTX127948
Anti-OPG antibody used in Western Blot (WB). GTX127948

Cat No. GTX127948

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all OPG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

human plasma , TNFRSF11B-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human OPG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885664

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 11b , OCIF , OPG , PDB5 , TR1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Database

  • Gene ID: 4982 TNFRSF11B
  • UniProt: O00300 TNFRSF11B

Research Area

DATA IMAGES

Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX127948

GTX127948 IHC-P Image

OPG antibody detects TNFRSF11B protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
OPG antibody (GTX127948) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ryuta Sumida et al. Experimental and Therapeutic Medicine 2019; : 358-365 Platelet?rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor?κB ligand ratio in osteoblasts.
Application : WB
Reactivity : Mouse
Chang MC et al. Int J Mol Sci 2018; 19 (12) Butyrate Stimulates Histone H3 Acetylation, 8-Isoprostane Production, RANKL Expression, and Regulated Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Mei-Chi Chang et al. pharmacology & toxicology 2018; (Epub) Butyrate Stimulates Histone H3 Acetylation, RANKL Expression, But Inhibited Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169