GeneTex

  • Country / Location Selection

United States (US)

OPG antibody

Orthogonal Overexpression
Anti-OPG antibody used in Western Blot (WB). GTX127948
Anti-OPG antibody used in Western Blot (WB). GTX127948
Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX127948
Anti-OPG antibody used in Western Blot (WB). GTX127948
Anti-OPG antibody used in Western Blot (WB). GTX127948

Cat. No. GTX127948

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OPG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

TNFRSF11B-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human OPG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885664

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor superfamily member11b , OCIF , OPG , PDB5 , TNFRSF11B , TR1 , Osteoprotegerin , osteoclastogenesis inhibitory factor

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Database

  • Gene ID: 4982 TNFRSF11B
  • UniProt: O00300 TNFRSF11B

Research Area

DATA IMAGES

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX127948

GTX127948 IHC-P Image

OPG antibody detects TNFRSF11B protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
OPG antibody (GTX127948) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OPG antibody used in Western Blot (WB). GTX127948

GTX127948 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

CW Park et al. Research Square 2023; Targeting Bone Marrow Lipid Metabolism with Adiponectin Receptor Agonist for Diabetic and Postmenopausal Osteoporosis
Application : WB, IHC-P
Hu-Qiang He et al. Front Pharmacol 2021; 12 : 692431 AGEs-Induced Calcification and Apoptosis in Human Vascular Smooth Muscle Cells Is Reversed by Inhibition of Autophagy.
Application : WB
Reactivity : Human
Ryuta Sumida et al. Experimental and Therapeutic Medicine 2019; : 358-365 Platelet?rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor?κB ligand ratio in osteoblasts.
Application : WB
Reactivity : Mouse
Chang MC et al. Int J Mol Sci 2018; 19 (12) Butyrate Stimulates Histone H3 Acetylation, 8-Isoprostane Production, RANKL Expression, and Regulated Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Mei-Chi Chang et al. pharmacology & toxicology 2018; (Epub) Butyrate Stimulates Histone H3 Acetylation, RANKL Expression, But Inhibited Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for OPG antibody (GTX127948). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169