Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ORP1 antibody [C3], C-term (GTX107722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunofluorescence analysis of methanol-fixed HeLa, using ORP1(GTX107722) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1(GTX107722) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX107722 diluted at 1:1000