GeneTex
United States (US)

ORP150 antibody [C2C3], C-term

GTX102255_39785_WB_R_brain_19011506_401.jpg
GTX102255_39785_20150410_IFA_19011506_531.jpg
GTX102255_39785_IFA_19011506_899.jpg
GTX102255_39785_WB_19011506_526.jpg
GTX102255_39785_WB_M_liver_19011506_704.jpg
GTX102255_39785_20150410_IFA_2_19011506_232.jpg
GTX102255_39785_IHC_19011506_889.jpg
GTX102255_39785_20141231_IHC_M_2_19011506_399.jpg

Cat No. GTX102255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Plant
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

111 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , mouse liver , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxia Up-Regulated 1,Grp-170,Grp170,Hsp12A,Orp-150,Orp150,Hyou1

Cellular Localization

Endoplasmic reticulum lumen

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102255_39785_WB_R_brain_19011506_401.jpg

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255_39785_20150410_IFA_19011506_531.jpg

GTX102255 ICC/IF Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX102255_39785_IFA_19011506_899.jpg

GTX102255 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using ORP150(GTX102255) antibody at 1:500 dilution.

GTX102255_39785_WB_19011506_526.jpg

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255_39785_WB_M_liver_19011506_704.jpg

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255_39785_20150410_IFA_2_19011506_232.jpg

GTX102255 ICC/IF Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102255_39785_IHC_19011506_889.jpg

GTX102255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ORP150(GTX102255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102255_39785_20141231_IHC_M_2_19011506_399.jpg

GTX102255 IHC-P Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf