ORP150 antibody [C2C3], C-term

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ORP150(GTX102255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed Hep3B, using ORP150(GTX102255) antibody at 1:500 dilution.

ORP150 antibody detects ORP150 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ORP150 antibody detects ORP150 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ORP150 antibody [C2C3], C-term detects ORP150 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

The data was published in the journal Front Neurol in 2019.PMID: 31258504

The data was published in the 2022 in Orphanet J Rare Dis. PMID: 35101074