Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody [HL3673] (GTX641701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody [HL3673] (GTX641701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ORP8 antibody [HL3673] detects ORP8 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ORP8 stained by ORP8 antibody [HL3673] (GTX641701) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min