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Oct4 antibody [GT486]

Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

Cat No. GTX627419

Host Mouse
Clonality Monoclonal
Clone Name GT486
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:1000-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NT2D1 , human ESC , mouse ESC , HeLa transfected recombinant OCT3/4 protein

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 5 Homeobox 1,Oct3,Oct4,Otf-3,Otf3,Otf4,Oct-3,Oct-4,Pou5F1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419

GTX627419 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Sample (10 µg of whole cell lysate)
A: Non-transfected HeLa lysates
B: HeLa transfected recombinant Oct4 protein
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419

GTX627419 FACS Image

Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Sample (30 µg of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Red: phalloidin, a cytoskeleton  marker, diluted at 1:100.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.
A. 30 µg human ESC whole cell lysate/extract
10% SDS-PAGE
(GTX627419) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Oct4 antibody [GT486] Cat No. GTX627419

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Date : Anonymous submitted on 16-May-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169