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Oct4 antibody [GT486]

Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

Cat No. GTX627419

Host

Mouse

Clonality

Monoclonal

Clone Name

GT486

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:1000-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NT2D1 , human ESC , mouse ESC , HeLa transfected recombinant OCT3/4 protein

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11176459

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419

GTX627419 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Sample (10 μg of whole cell lysate)
A: Non-transfected HeLa lysates
B: HeLa transfected recombinant Oct4 protein
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419

GTX627419 FACS Image

Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
(GTX627419) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Mouse embryonic stem cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

REVIEW

Oct4 antibody [GT486] Cat No. GTX627419

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019.PMID: 31878324
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Date : Anonymous submitted on 16-May-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169