GeneTex
United States (US)

Oct4 antibody

GTX101497_40254_IP_19011506_698.jpg
GTX101497_40254_CT_IFA_hESC2_19011506_586.jpg
GTX101497_40254_CT_IFA_mESC2_19011506_785.jpg
GTX101497_40254_WB_M_ESC_19011506_201.jpg
GTX101497_40254_IHC_19011506_784.jpg
GTX101497_42354_20160602_IFA_19011506_163.jpg
GTX101497_41731_WB_ESC_19011506_354.jpg
GTX101497_41899_20160428_WB_19011506_892.jpg
GTX101497_40254_CT_FACS_19011506_191.jpg

Cat No. GTX101497

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human ESC , mouse ESC , NT2D1

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC22487 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, POU5F1 antibody, POU-type homeodomain-containing DNA-binding protein antibody, octamer-binding protein 3 antibody, POU domain transcription factor OCT4 antibody, octamer-binding protein 4 antibody, POU domain, class 5, transcription factor 1 antibody, octamer-binding transcription factor 3 antibody, octamer-binding transcription factor-3 antibody, POU class 5 homeobox 1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101497_40254_IP_19011506_698.jpg

GTX101497 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101497_40254_CT_IFA_hESC2_19011506_586.jpg

GTX101497 ICC/IF Image

Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

GTX101497_40254_CT_IFA_mESC2_19011506_785.jpg

GTX101497 ICC/IF Image

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

GTX101497_40254_WB_M_ESC_19011506_201.jpg

GTX101497 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101497_40254_IHC_19011506_784.jpg

GTX101497 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OCT3/4(GTX101497) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101497_42354_20160602_IFA_19011506_163.jpg

GTX101497 ICC/IF Image

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101497_41731_WB_ESC_19011506_354.jpg

GTX101497 WB Image

Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 µg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101497_41899_20160428_WB_19011506_892.jpg

GTX101497 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

GTX101497_40254_CT_FACS_19011506_191.jpg

GTX101497 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169