APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:100000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:50-1:200 |
1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse ESC , human ESC , NT2D1
Predict Reactivity
Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618784
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101497 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101497 ICC/IF Image
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX101497 ICC/IF Image
Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
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GTX101497 FACS Image
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).
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GTX101497 WB Image
Sample (20 μg of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101497 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 IHC-P Image
Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101497 ICC/IF Image
Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
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GTX101497 WB Image
Oct4 antibody detects Oct4 protein by western blot analysis. A. 30 μg human ESC whole cell extract 12% SDS-PAGE Oct4 antibody (GTX101497) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.
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GTX101497 WB Image
The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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GTX101497 IHC-P Image
The data was published in the journal BMC Cancer in 2019. PMID: 31399076
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GTX101497 WB Image
The data was published in the 2019 in Cancers (Basel). PMID: 31861872
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GTX101497 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26992205
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GTX101497 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26992205
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GTX101497 IHC-P Image
The data was published in the journal BMC Cancer in 2019. PMID: 31399076
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GTX101497 ICC/IF Image
The data was published in the journal Oncotarget in 2020.PMID: 32256979
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REFERENCE
REVIEW
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