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Oct4 antibody

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497

Cat No. GTX101497

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human ESC , mouse ESC , NT2D1

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618784

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497

GTX101497 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497

GTX101497 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

REFERENCE

REVIEW

Oct4 antibody Cat No. GTX101497

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2020.PMID: 32256979
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2019.PMID: 31399076
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2015.PMID: 26136074
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2019.PMID: 31399076
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 26992205
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 26992205
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf