APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:100000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:50-1:200 |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse ESC , human ESC , NT2D1
Predict Reactivity
Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.49 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618784
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101497 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101497 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 ICC/IF Image
Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
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GTX101497 ICC/IF Image
Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
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GTX101497 WB Image
Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 ICC/IF Image
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX101497 WB Image
Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101497 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.
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GTX101497 FACS Image
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).
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GTX101497 IHC-P Image
Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
Oct4 antibody
Cat. No. GTX101497
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Rating
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( Average 5 based on 6 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 3 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2020.
PMID: 32256979
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal BMC Cancer in 2019.
PMID: 31399076
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2015.
PMID: 26136074
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Figure Note :
The data was peer reviewed and published in the journal BMC Cancer in 2019.
PMID: 31399076
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 26992205
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 26992205
