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Oct4 antibody

KO/KD Orthogonal Overexpression
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

Cat. No. GTX101497

Reference 34

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Oct4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse ESC , human ESC , NT2D1

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618784

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497

GTX101497 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497

GTX101497 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26992205

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26992205

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Orthogonal

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

Overexpression

The data was published in the journal BMC Cancer in 2019. PMID: 31399076

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

The data was published in the journal Oncotarget in 2020.PMID: 32256979

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

The data was published in the journal BMC Cancer in 2019. PMID: 31399076

REFERENCE

Application Reference

J Liu et al. Trop J Pharm Res 2024; 23 (1) : 27-35
Application : WB
S H?pperger et al. Stem Cell Res 2024; 75 : 103321 Generation of the human induced pluripotent stem cell line (IBKMOLi003-A) from PBMCs of a vascular Ehlers-Danlos syndrome (vEDS) patient carrying the heterozygous nonsense mutation c.430C > T (p.Q105*) in the COL3A1 gene
YF Tsai et al. Cancer Cell Int 2023; RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma
Application : WB
Wang CH et al. Regen Ther 2023; 24 : 443-450 Cbx7 promotes the generation of induced pluripotent stem cells.
CH Wang et al. Regen Ther 2023; 24 : 443-450 Cbx7 promotes the generation of induced pluripotent stem cells
Application : ICC/IF
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REVIEW

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Application Reference
J Liu et al. Trop J Pharm Res 2024; 23 (1): 27-35
Application : WB
S H?pperger et al. Stem Cell Res 2024; 75:103321 Generation of the human induced pluripotent stem cell line (IBKMOLi003-A) from PBMCs of a vascular Ehlers-Danlos syndrome (vEDS) patient carrying the heterozygous nonsense mutation c.430C > T (p.Q105*) in the COL3A1 gene
YF Tsai et al. Cancer Cell Int 2023; RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma
Application : WB
Wang CH et al. Regen Ther 2023; 24:443-450 Cbx7 promotes the generation of induced pluripotent stem cells.
CH Wang et al. Regen Ther 2023; 24:443-450 Cbx7 promotes the generation of induced pluripotent stem cells
Application : ICC/IF
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Mohankumar K et al. Mol Carcinog 2022; 61 (1):73-84 Nuclear receptor 4A1 (NR4A1) antagonists target paraspeckle component 1 (PSPC1) in cancer cells.
Application : WB
Reactivity : Human
Peng Ye et al. Biomolecules 2022; 12 (5) Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.
Application : WB
Reactivity : Human
Ahmad Salti et al. Cells 2021; 10 (8) High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic Fibroblasts.
Application : ICC/IF
Reactivity : Mouse
Zhang W et al. Stem Cell Res 2021; 57:102611 Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.
Application : ICC/IF
Reactivity : Human
He Q et al. Stem Cell Res Ther 2021; 12 (1):449 Mesenchymal stem cell-derived exosomal miR-146a reverses diabetic β-cell dedifferentiation.
Application : ICC/IF
Reactivity : Rat
Fei Peng et al. Cell Res 2020; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Application : WB
Reactivity : Human
Deng H et al. Cell Transplant 2020; 29:963689720927392 Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.
Assoun EN et al. Oncotarget 2020; 11 (12):1075-1084 Characterization of iPS87, a prostate cancer stem cell-like cell line.
Wang YY et al. Cancers (Basel) 2020; 12 (12) Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.
Application : WB
Reactivity : Human
Fang W et al. Biomark Med 2020; 14 (15):1473-1484 Prognostic value of OCT4 in colorectal cancer: analysis using immunohistochemistry and bioinformatics validation.
Chiou HC et al. Neoplasia 2020; 22 (7):263-271 Stemness regulation of the adrenal mixed corticomedullary tumorigenesis-a case-control study.
Application : IHC-P
Reactivity : Human
Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Wang CH et al. Sci Rep 2020; 10 (1):2187 The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency.
Application : WB
Reactivity : Mouse
Brown RM et al. J Virol 2019; 93 (17) Human Cytomegalovirus Compromises Development of Cerebral Organoids.
Huang JY et al. Cancers (Basel) 2019; 12 (1) Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
Application : WB
Reactivity : Human
Ko HJ et al. J Clin Med 2019; 8 (10) GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease.
Application : ICC/IF
Reactivity : Human
Feng YH et al. BMC Cancer 2019; 19 (1):791 Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer.
Application : IHC-P
Reactivity : Human
Xuan X et al. Metabolism 2017; (Epub) Activation of ACE2/angiotensin (1-7) attenuates pancreatic β cell dedifferentiation in a high-fat-diet mouse model.
Application : IHC-P
Reactivity : Mouse
Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Emhemmed F et al. Cell Biol Toxicol 2016; (Epub) Pro-differentiating effects of a synthetic flavagline on human teratocarcinomal cancer stem-like cells.
Application : ICC/IF
Reactivity : Human
Chen MJ et al. Sci Rep 2016; 6:34933 PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Application : WB
Reactivity : Human
Lee TY et al. Oncotarget 2016; (Epub) Increased chemoresistance via snail-raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative.
Application : WB
Reactivity : Human
Mineda K et al. Stem Cells Transl Med 2015; 4 (12):1511-22 Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.
Application : IHC-Fr
Reactivity : Human
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
Application : WB
Reactivity : Human
Chen YK et al. Int J Oral Sci 2014; 6 (4):212-8 Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma.
Cheng NC et al. Stem Cells Transl Med 2013; 2 (8):584-94 Short-term spheroid formation enhances the regenerative capacity of adipose-derived stem cells by promoting stemness, angiogenesis, and chemotaxis.
Application : WB
Reactivity : Human
Cheng PH et al. Am J Hum Genet 2013; 93 (2):306-12 miR-196a ameliorates phenotypes of Huntington disease in cell, transgenic mouse, and induced pluripotent stem cell models.
Application : ICC/IF
Reactivity : Human
Cheng NC et al. Biomaterials 2012; 33 (6):1748-58 The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169