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PACSIN1 antibody

Anti-PACSIN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108567
Anti-PACSIN1 antibody used in Western Blot (WB). GTX108567
Anti-PACSIN1 antibody used in Western Blot (WB). GTX108567
Anti-PACSIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108567

Cat. No. GTX108567

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PACSIN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human PACSIN1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951092

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C and casein kinase substrate in neurons 1 , SDPI

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-PACSIN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108567

GTX108567 ICC/IF Image

PACSIN1 antibody detects PACSIN1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PACSIN1 protein stained by PACSIN1 antibody (GTX108567) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PACSIN1 antibody used in Western Blot (WB). GTX108567

GTX108567 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PACSIN1 antibody (GTX108567) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PACSIN1 antibody used in Western Blot (WB). GTX108567

GTX108567 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX108567 diluted at 1:500

Anti-PACSIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108567

GTX108567 IHC-P Image

PACSIN1 antibody detects PACSIN1 protein at cytoplasm on MCF7 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MCF7 xenograft.
PACSIN1 antibody (GTX108567) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169