Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
PAD4 antibody (GTX113945) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4(GTX113945) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.