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PAD4 antibody

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945
Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113945
Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945
Anti-PAD4 antibody used in Western Blot (WB). GTX113945
Anti-PAD4 antibody used in Western Blot (WB). GTX113945

Cat No. GTX113945

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

PAD4-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037599

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peptidyl arginine deiminase 4 , PAD , PAD4 , PADI5 , PDI4 , PDI5

Cellular Localization

Cytoplasm , Nucleus , Cytoplasmic granule

Background

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945

GTX113945 IHC-P Image

PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
PAD4 antibody (GTX113945) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113945

GTX113945 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4(GTX113945) antibody at 1:200 dilution.

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945

GTX113945 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4(GTX113945) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAD4 antibody used in Western Blot (WB). GTX113945

GTX113945 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAD4 antibody used in Western Blot (WB). GTX113945

GTX113945 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf