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PAF49 antibody [GT1964]

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115
Anti-PAF49 antibody [GT1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629115
Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115

Cat No. GTX629115

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1964

Isotype

IgG2b

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888097

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115

GTX629115 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629115) diluted at 1:500.

Anti-PAF49 antibody [GT1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629115

GTX629115 ICC/IF Image

CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (GTX629115) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115

GTX629115 WB Image

PAF49 antibody [GT1964] detects PPY protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PAF49 antibody [GT1964] (GTX629115) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169