GeneTex
United States (US)

PAK4 (Active) recombinant protein

Cat No. GTX65316

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

83 nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

~90.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine/threonineprotein kinase, PAK, PAK4, Serine/threonine-protein kinase

Background

PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells. PAK4 regulates the activity of LIM kinase 1 which in turn phosphorylates Cofilin leading to cytoskeletal changes. PAK4 can protect cells from apoptosis in response to several different types of stimuli by inhibiting the pro-apoptotic proteins Bad and Caspase 8. PAK4 has been shown to associate with and mediate the downstream signaling of the keratinocyte growth factor receptor. In addition, PAK4 interacts with the beta5 integrins and regulates cell migration during engagement of the alpha vbeta5 integrin receptor.

Research Area

Package List Price ($)
$ 349