Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PARP antibody [GT6212] detects PARP protein at nucleus in human Hep G2 by immunohistochemical analysis.
Sample: Paraffin-embedded human Hep G2.
PARP antibody [GT6212] (GTX628836) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30μM Cisplatin treatment for 24hr)
7.5 % SDS-PAGE
PARP1 antibody [GT6212] (GTX628836) dilution: 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.