Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:50000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PARP1 antibody detects PARP1 protein by western blot analysis. Un-treated (-) and treated (+, 30uM cisplatin treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody (GTX632388) at a dilution of 1:1000.

Immunoprecipitation of PARP protein from 293T whole cell extracts using 5 μg of PARP antibody (GTX632388).
Western blot analysis was performed using PARP antibody (GTX632388).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

PARP1 antibody [GT982] detects PARP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP1 protein stained by PARP1 antibody [GT982] (GTX632388) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.