APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , HCT116 , HCT116 (30 μM cisplatin treatment for 24 hr) , NIH-3T3 , PC-12 , *H1299
Specificity/Sensitivity
IP/MS validation was supported by references (PMID:30377409)
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.36mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ADPRT antibody, ADPRT 1 antibody, ADPRT1 antibody, PARP antibody, PARP-1 antibody, PPOL antibody, pADPRT-1 antibody, PARP1 antibody, poly[ADP-ribose] synthase 1 antibody, poly(ADP-ribosyl)transferase antibody, poly(ADP-ribose) polymerase antibody, poly(ADP-ribose) synthetase antibody, poly (ADP-ribose) polymerase family, member 1 antibody, ADP-ribosyltransferase NAD(+) antibody, NAD(+) ADP-ribosyltransferase 1 antibody, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) antibody, poly [ADP-ribose] polymerase 1 antibody, poly (ADP-ribose) polymerase 1 antibody
Cellular Localization
Nucleus
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112864 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 IP Image
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864) 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX112864 ChIP assay Image
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.
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GTX112864 IHC-P Image
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112864 WB Image
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX112864 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 WB Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 ICC/IF Image
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP protein stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX112864 WB Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX112864 ChIP assay Image
ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
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GTX112864 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
PARP antibody [N2C1], Internal
Cat No. GTX112864
Rating
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( Average 4 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 24-Jul-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
H1299 cells
Amount used :
30μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 24Hr