PRODUCT
Summary
PARP antibody (more specifically, PARP1 antibody) detects poly(ADP-ribose) polymerase (PARP)-1, which is involved in various biological mechanisms such as DNA repair, genomic stability, and programmed cell death. Preclinical and clinical studies showed that PARP inhibition is an effective anti-tumor modality, and thus PARP inhibitors have become essential components of various chemotherapeutic regimens against many malignancies.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3,293T,A431,HeLa,HepG2,293T
Product Note
This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.
IP/MS validation was supported by references (PMID:30377409)
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11173565
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1
Cellular Localization
Nucleus,Nucleolus
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX112864 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX112864 IHC-P Image
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112864 ChIP assay Image
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
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GTX112864 IHC-P Image
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112864 IP Image
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864) 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX112864 IHC-P Image
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112864 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 ChIP assay Image
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.
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GTX112864 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 ICC/IF Image
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX112864 WB Image
Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112864 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX112864 WB Image
The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384
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REFERENCE
REVIEW
PARP antibody [N2C1], Internal
Cat. No. GTX112864
Rating
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( Average 4.5 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 31-Jan-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
iPSC
Amount used :
25 μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Date :
Anonymous submitted on 24-Jul-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
H1299 cells
Amount used :
30μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 24Hr