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PARP antibody [N2C1], Internal

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112864
Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864
Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864
Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX112864
Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

Cat. No. GTX112864

Reference 10
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PARP products
PRODUCT

Summary

PARP antibody (more specifically, PARP1 antibody) detects poly(ADP-ribose) polymerase (PARP)-1, which is involved in various biological mechanisms such as DNA repair, genomic stability, and programmed cell death. Preclinical and clinical studies showed that PARP inhibition is an effective anti-tumor modality, and thus PARP inhibitors have become essential components of various chemotherapeutic regimens against many malignancies.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12 , 293T , A431 , HeLa , HepG2 , 293T

Product Note

IP/MS validation was supported by references (PMID:30377409)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173565

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112864

GTX112864 ICC/IF Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864

GTX112864 IHC-P Image

PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864

GTX112864 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864

GTX112864 IHC-P Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX112864

GTX112864 IP Image

PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864

GTX112864 IHC-P Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864

GTX112864 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Jang M et al. Cancers (Basel) 2021; 13 (16) LNX1 Contributes to Cell Cycle Progression and Cisplatin Resistance.
Application : WB
D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17) : E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Show More +
Submit a Reference

REVIEW

PARP antibody [N2C1], Internal Cat. No. GTX112864

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2021 in Cancer Drug Resist. PMID: 35582384
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Date : Anonymous submitted on 31-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : iPSC
Amount used : 25 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 24Hr
Submit a Review
Application Reference
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Jang M et al. Cancers (Basel) 2021; 13 (16) LNX1 Contributes to Cell Cycle Progression and Cisplatin Resistance.
Application : WB
D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17):E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Kim SH et al. Biomedicine & Pharmacotherapy 2018; 99:997-1008 Teroxirone suppresses growth and motility of human hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Chien T et al. Mol Psychiatry 2018; (Epub) GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
Application : WB
Reactivity : Rat
Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Buchkremer S et al. JND 2016; 3 (2):169-181 Sil1-Mutant Mice Elucidate Chaperone Function in Neurological Disorders.
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169