GeneTex
United States (US)

PARP antibody

KO/KD Comparison Orthogonal
GTX100573_39596_WB_cisplatin_19011506_589.jpg
GTX100573_39596_20160623_WB_shRNA_watermark_19011506_246.jpg
GTX100573_42711_20171222_WB_competitor_watermark_19011506_645.jpg
GTX100573_42712_20170315_IFA_19011506_188.jpg
GTX100573_42712_20170601_IHC-P_19011506_392.jpg
GTX100573_39596_ChIP_19011506_502.jpg
GTX100573_42711_20170126_WB_19011506_418.jpg
GTX100573_42711_20171103_WB_R_19011506_556.jpg
GTX100573_42711_20170420_WB_cisplatin_19011506_132.jpg

Cat No. GTX100573

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HeLa (30 μM cisplatin treatment for 24 hr) , HCT116 , HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 ,

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Poly(Adp-Ribose) Polymerase 1,Adprt,Adprt 1,Adprt1,Artd1,Parp,Parp-1,Ppol,Padprt-1,Parp1

Cellular Localization

Nucleus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100573_39596_WB_cisplatin_19011506_589.jpg

GTX100573 WB Image

Orthogonal

PARP1 antibody detects PARP1 protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract (untreated)
B. 30 µg HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr)
7.5% SDS-PAGE
PARP1 antibody (GTX100573) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100573_39596_20160623_WB_shRNA_watermark_19011506_246.jpg

GTX100573 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100573_42711_20171222_WB_competitor_watermark_19011506_645.jpg

GTX100573 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody (GTX100573) diluted at 1:2000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100573_42712_20170315_IFA_19011506_188.jpg

GTX100573 ICC/IF Image

PARP antibody detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP protein stained by PARP antibody (GTX100573) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

GTX100573_42712_20170601_IHC-P_19011506_392.jpg

GTX100573 IHC-P Image

PARP antibody detects PARP protein at nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
PARP antibody (GTX100573) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100573_39596_ChIP_19011506_502.jpg

GTX100573 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.

GTX100573_42711_20170126_WB_19011506_418.jpg

GTX100573 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000.

GTX100573_42711_20171103_WB_R_19011506_556.jpg

GTX100573 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100573_42711_20170420_WB_cisplatin_19011506_132.jpg

GTX100573 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

PARP antibody Cat No. GTX100573

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169