PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [GT9412] (GTX634863) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.