ICC/IF analysis of MCF-7 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

WB analysis of 50 μg of various whole cell lysates using GTX54533 PAX7 antibody.
Dilution : 1:1000

ICC/IF analysis of C2C12 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IP analysis of HeLa cell lysates using GTX54533 PAX7 antibody.
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:1000

WB analysis of 50μg of HeLa lysate overexpressing PAX7 (right lane) or empty vector control (left lane) using GTX54533 PAX7 antibody.
Dilution : 1:1000

ICC/IF analysis of MCF7 and HeLa cells using GTX54533 PAX7 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature