GeneTex
United States (US)

PCBP2 antibody

Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX33389
Anti-PCBP2 antibody used in Western Blot (WB). GTX33389
Anti-PCBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33389

Cat No. GTX33389

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

39 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PCBP2 (NP_001122383.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Poly(Rc) Binding Protein 2,Hnrnpe2,Hnrpe2,Hnrnp-E2,Pcbp2

Background

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX33389

GTX33389 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33389 PCBP2 antibody.
Dilution : 1:100

Anti-PCBP2 antibody used in Western Blot (WB). GTX33389

GTX33389 WB Image

WB analysis of various samples using GTX33389 PCBP2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PCBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33389

GTX33389 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33389 PCBP2 antibody.
Blue : DAPI

Package List Price ($)
$ 329