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PCK1 antibody

Anti-PCK1 antibody used in Western Blot (WB). GTX54629
Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629
Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629
Anti-PCK1 antibody used in Immunoprecipitation (IP). GTX54629
Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629

Cat. No. GTX54629

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
IHC-P Assay dependent
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human PCK1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 1 , PCKDC , PEPCK-C , PEPCK1 , PEPCKC

Cellular Localization

Cytoplasm

Background

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCK1 antibody used in Western Blot (WB). GTX54629

GTX54629 WB Image

WB analysis of various sample lysates using GTX54629 PCK1 antibody.
Loading : 25μg per lane

Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629

GTX54629 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54629 PCK1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629

GTX54629 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54629 PCK1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCK1 antibody used in Immunoprecipitation (IP). GTX54629

GTX54629 IP Image

IP analysis of mouse liver tissue lysate using GTX54629 PCK1 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-PCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54629

GTX54629 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX54629 PCK1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399