Immunohistochemical analysis of paraffin-embedded human breast cancer, using PCK2(GTX114919) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody (GTX114919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PCK2 antibody detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in MeOH for 5 min.
Green: PCK2 protein stained by PCK2 antibody (GTX114919) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Oncotarget in 2017. PMID: 29262588