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PCK2 antibody

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919

Cat No. GTX114919

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Neuro2A , GL261 , PC-12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial,PEPCK,PEPCK-M,PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919

GTX114919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PCK2(GTX114919) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody (GTX114919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919

GTX114919 ICC/IF Image

PCK2 antibody detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in MeOH for 5 min.
Green: PCK2 protein stained by PCK2 antibody (GTX114919) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169