GeneTex
United States (US)

PDGF-AA recombinant protein

Cat No. GTX65194

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1x10^6 units/mg.

Calculated MW

28.5 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitution in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Osteosarcoma-derived Growth Factor, PDGF1., Gliomaderived growth factor, PDGFAA, PDGFA, PDGF-1., Osteosarcomaderived Growth Factor, Glioma-derived growth factor, GDGF, ODGF, PDGF-AA, PDGF 1., PDGF AA, Glioma derived growth factor

Background

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.

Research Area