Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PDGF Receptor alpha antibody [N2C2], Internal detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)

A: K562

5% SDS PAGE

GTX107903 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.

Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.