WB analysis of NIH3T3 stimulated with 50ng/mL PDGF-BB for 10 mins (Lane 2-5) using GTX25443 PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine containing peptide(Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of MCF7 cells treated with PDGF (50 ng/mL for 10 min) using GTX25443 PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes