GeneTex
United States (US)

PDI (Active) recombinant protein

Cat No. GTX65193

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Recombinant PDI is biological active. Reductase activity is 1.0 x 10e3 ∆650nm/ min-2, by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase activity is 0.5 umol active RNase A min-1 umol PDI-1, according to the re-activation of reduced and denatured RNase A.

Calculated MW

57.1 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstituted PDI should be stored in working aliquots at -20ºC. For long-term storage, it is recommended to add carrier protein (0.1% HAS or BSA).

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Disulfide Isomerase

Background

Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.

Research Area

Package List Price ($)
$ 259