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PER2 antibody

Anti-PER2 antibody used in Western Blot (WB). GTX134478

Cat No. GTX134478

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

PER2-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse PER2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887292

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

period circadian clock 2 , mKIAA0347 , mPer2

Cellular Localization

Nucleus,Cytoplasm

Background

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-PER2 antibody used in Western Blot (WB). GTX134478

GTX134478 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX134478) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

PER2 antibody Cat No. GTX134478

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 24-Jan-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brain tissue lysate
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169