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PERK antibody

Orthogonal
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275
Anti-PERK antibody used in Western Blot (WB). GTX129275

Cat. No. GTX129275

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PERK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PERK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 3 , PEK , PERK , WRS

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275

GTX129275 IHC-P Image

PERK antibody detects PERK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PERK stained by PERK antibody (GTX129275) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275

GTX129275 IHC-P Image

PERK antibody detects PERK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PERK stained by PERK antibody (GTX129275) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PERK antibody used in Western Blot (WB). GTX129275

GTX129275 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PERK antibody (GTX129275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Piper JA et al. Molecules 2023; 28 (12) L-Proline Prevents Endoplasmic Reticulum Stress in Microglial Cells Exposed to L-azetidine-2-carboxylic Acid.
Xiaofeng G et al. CNS Neurosci Ther 2023; 29 (4) : 1178-1191 PERK-STING-RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis-associated encephalopathy.
Application : WB
Reactivity : Mouse
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

PERK antibody Cat. No. GTX129275

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-PERK antibody used in Western Blot (WB). GTX129275
Date : Anonymous submitted on 05-Jan-2022
Score :
Application Tested : WB
Sample Species : Rat
Sample : A10 cells
Amount used : 60μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-PERK antibody used in Western Blot (WB). GTX129275
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM DOX treatment for 2 h.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169