Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX114875 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114875 diluted at 1:1000

VBP1 antibody detects VBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VBP1 protein stained by VBP1 antibody (GTX114875) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.