PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.