Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [GT21612] (GTX634447) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.