Wild-type (WT) and PGP9.5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PGP9.5 antibody [GT448] detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500.
Red: NeuN, a neuronal nuclei marker, stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PGP9.5 antibody [GT448] detects PGP9.5 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

PGP9.5 antibody [GT448] detects PGP9.5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min