Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PHACTR3 antibody (GTX119979) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHACTR3 antibody (GTX119979) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.