Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody [HL4042] (GTX642457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody [HL4042] (GTX642457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.