Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PIKfyve antibody [HL3449] (GTX641322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PIKfyve antibody [HL3449] (GTX641322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PIKfyve antibody [HL3449] (GTX641322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.