GeneTex
United States (US)

PIM2 (Active) recombinant protein

Cat No. GTX65323

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

305 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

61.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine/threonine-protein kinase Pim-2, PIM, PIM2, Serine/threonineprotein kinase Pim2

Background

Baytel et al. identified a deduced 334-amino acid sequence of the clone, which showed 90% identity with the 22 Pim2 protein. Like 22 Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. The results suggested a role for PIM2 in proliferating cells as well as during meiosis (1). Yan et al investigated potential functions for the pim family of kinases in factor-dependent murine hematopoietic cells and indicate that pim-2 functions similarly to pim-1 as a pro-survival kinase and suggest that BAD is a legitimate PIM-2 substrate (2). Hammerman et al concluded that the transcriptional induction of Pim-2 initiated a novel NF-kappaB activation pathway that regulates cell survival (3).

Research Area