Dot blot analysis of GST and GST-fusion proteins using GTX80257 PKA C alpha antibody [6D2.1] and GTX79895 GST tag antibody [S-tag-05]. The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1 : GST-Akt1
Lane 2 : GST-Akt2
Lane 3 : GST-Akt3
Lane 4 : GST-PKAc alpha
Lane 5 : GST-PKAc beta
Lane 6 : GST-PKAc gamma
Lane 7 : GST-MEK 1
Lane 8 : GST