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PKA C alpha antibody

Anti-PKA C alpha antibody used in Western Blot (WB). GTX19242

Cat No. GTX19242

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat, Bovine
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-4 μg/ml
IP 20-30 μg/ml
Not tested in other applications.

Calculated MW

40.59 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 30% glycerol, 0.5% BSA, 0.01% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide surrounding amino acid 11 of the catalytic subunit of human PKA alpha isoform

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PKACA , PPNAD4 , PRKACA , protein kinase cAMPactivated catalytic subunit alpha , PKA C alpha , protein kinase cAMP-activated catalytic subunit alpha

Cellular Localization

Cytoplasm,Cell membrane,Nucleus,Mitochondrion,Membrane,Cell projection,Cilium,Flagellum,Cytoplasmic vesicle,Secretory vesicle,Acrosome

Background

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES
Anti-PKA C alpha antibody used in Western Blot (WB). GTX19242

GTX19242 WB Image

WB analysis of Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3), and rat kidney (Lane 4) lysates using GTX19242 PKA C alpha antibody.

REFERENCE
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Package List Price ($)
$ 329