GeneTex
United States (US)

PKA R2 antibody [H2 006 D4]

Orthogonal
GTX16283_7_ChIP_19011519_647.jpg
GTX16283_1551_WB_19011519_686.jpg

Cat No. GTX16283

Host Mouse
Clonality Monoclonal
Clone Name H2 006 D4
Isotype IgG2b
Application WB, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ChIP assay 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

This antibody does not discriminate between RII alpha and beta.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Column purified fraction of cAMP dependent PKA RII from human saliva.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase Camp-Dependent Type Ii Regulatory Subunit Alpha,Pkr2,Prkar2,Prkar2A

Cellular Localization

Cell membrane,Cytoplasm

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX16283_7_ChIP_19011519_647.jpg

GTX16283 ChIP assay Image

Orthogonal

ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 µg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX16283 PKA R2 antibody [H2 006 D4]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene
IP reaction : 1.0ul antibody / 100ul sample

GTX16283_1551_WB_19011519_686.jpg

GTX16283 WB Image

WB analysis of human saliva using GTX16283 PKA R2 antibody [H2 006 D4].

Package List Price ($)
$ 319