WB (peptide competition) analysis of Y1 Adrenocortical cell lysates treated with phosphatase (Lane 5) using GTX25815 PKA alpha + beta (phospho Thr197) antibody prior incubated with the non-phosphopeptide (Lane 2), a generic (pT) peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.