United States (US)

PKC beta 2 (Active) recombinant protein

Cat No. GTX65210

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human

Application Note

339 nmol phosphate incorporated into CREBtide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

~105.0 kDa. ( Note )




50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol


Store at -80ºC. Product is stable for at least 6-12 months.


0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species


Expression System

Baculovirus (Sf9 insect cells)


Purity was assessed by SDS-PAGE (≥79%) and by HPLC.


For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


PRKCB, Protein kinase C beta II, PKC


Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Greenham determined the genomic structure of the PRKCbeta gene, which consists of 18 exons spanning 375 kb (1). PRKCbeta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Leitges found that the 2 isoforms, PRKCbeta1 and PRKCbeta2, play an important role in B-cell activation and may be functionally linked to Bruton tyrosine kinase in antigen receptor-mediated signal transduction (2). Su proposed that PRKCbeta inhibitors and inhibitors of other PRKC isoforms may be effective in treating disorders characterized by dysregulated NFKB survival signaling (3). Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress (4).

Research Area